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Literature summary for 4.1.99.22 extracted from

  • Kruse, I.; Maclean, A.E.; Hill, L.; Balk, J.
    Genetic dissection of cyclic pyranopterin monophosphate biosynthesis in plant mitochondria (2018), Biochem. J., 475, 495-509 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.22

Cloned(Commentary)

Cloned (Comment) Organism
gene CNX2, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes CNX2, i.e. 2065 genome-wide SNPs and 54 within the mapping interval of chromosome 2, viable allele of cnx2 in Arabidopsis thaliana Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information generation of cnx2 mutants by T-DNA insertion, line cnx2-1, and point mutation, line cnx2-2. Mutation mapping by whole genome sequencing, phenotype analyses Arabidopsis thaliana
R88Q mutant xd22 has a mutation in CNX2 affecting the proximal FeS cluster loop. Complementation of the cnx2-2 phenotype by a genomic fragment including the promoter region from position -1224 and UTRs, amplified by PCR. Mutant cnx2-2 plants are transformed using Agrobacterium tumefaciens strain GV3101. The cnx2-2 mutation is not a knockout allele and growth is rescued by ammonia. Crosses between heterozygous cnx2-1 CNX2 and homozygous cnx2-2 plants give F1 offspring in two phenotype categories: seedlings with wild-type appearance corresponding to cnx2-1 CNX2 and small chlorotic seedlings that are genotyped as cnx2-1 cnx2-2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Arabidopsis thaliana 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ CNX2/MOCS1A is an FeS enzyme depending on two Fe4S4 clusters, which are assembled by the mitochondrial FeS cluster assembly pathway Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + S-adenosyl-L-methionine + reduced electron acceptor Arabidopsis thaliana
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?
GTP + S-adenosyl-L-methionine + reduced electron acceptor Arabidopsis thaliana Col-0
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q39055
-
-
Arabidopsis thaliana Col-0 Q39055
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + S-adenosyl-L-methionine + reduced electron acceptor
-
 Arabidopsis thaliana (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?
GTP + S-adenosyl-L-methionine + reduced electron acceptor
-
 Arabidopsis thaliana Col-0 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
-
 ?

Synonyms

Synonyms Comment Organism
AT2G31955
-
 Arabidopsis thaliana
cnx2
-
 Arabidopsis thaliana
CNX2/MOCS1A
-
 Arabidopsis thaliana
MOCS1A
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
Fe-S center CNX2/MOCS1A is an FeS enzyme depending on two Fe4S4 clusters, which are assembled by the mitochondrial FeS cluster assembly pathway Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction cyclic pyranopterin monophosphate (cPMP) is strongly decreased in cnx2 mutants. Phenotype of T-DNA insertion mutant line cnx2-1, the cnx2-1 knockout is seedling-lethal but growth is rescued by ammonia, overview. Mutant xd22 has a mutation in CNX2 affecting the proximal FeS cluster loop. With hypoxanthine as a substrate, no activity is detectable in leaves from cnx2-2 plants. Nitrate reductase activity in leaf extract from cnx2-2 is 55% compared to wild-type values. Genetic complementation and strongly decreased activities of Moco enzymes show that the mutation in xd22 (R88Q) is located in CNX2, line cnx2-2, encoding the first step in Moco biosynthesis. The cnx2-2 mutation is not a knockout allele and growth is rescued by ammonia. Crosses between heterozygous cnx2-1 CNX2 and homozygous cnx2-2 plants give F1 offspring in two phenotype categories: seedlings with wild-type appearance corresponding to cnx2-1 CNX2 and small chlorotic seedlings that are genotyped as cnx2-1 cnx2-2 Arabidopsis thaliana
metabolism molybdenum cofactor (Moco) biosynthesis starts with the condensation of GTP into cyclic pyranopterin monophosphate (cPMP) by the consecutive action of GTP 3',8-cyclase and cPMP synthase, encoded by CNX2 and CNX3 in plants, respectively, pathways for FeS cluster assembly and Moco biosynthesis in Arabidopsis thaliana, overview Arabidopsis thaliana